Peer-reviewed Publications
A screening-compatible live cell fluorescence energy transfer-based assay for modulation of Rho GTPase activity
Müller PM, Rocks O
STAR Protoc, 2022, Dec;16(3)
Systems-analysis of RhoGEF/RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions
Müller PM, Rademacher J, Bagshaw RD, Wortmann C, Barth C, van Unen J, Alp KM, Guidice G, Eccles RL, Heinrich LE, Pascual-Vargas P, Sanchez-Castro M, Brandenburg L, Mbamalu G, Tucholska M, Spatt L, Wortmann C, Czajkowski MT, Welke RW, Zhang S, Nguyen V, Rrustemi T, Trnka P, Freitag K, Larsen B, Popp O, Mertins P, Gingras AC, Roth FP, Colwill K, Bakal C, Pertz O, Pawson T, Petsalaki E, Rocks O
Nat Cell Biology, 2020, Apr;22(4):498-511 F1000 recommended
Interaction modulation through arrays of clustered methyl-arginine protein modifications
Woodsmith J, Casado-Medrano V, Benlasfer N, Eccles RL, Hutten S, Heine CL, Thormann V, Abou-Ajram C, Rocks O, Dormann D, Stelzl U
Life Sci Alliance, 2018 Sep 21;1(5)
An AKAP-Lbc-RhoA interaction inhibitor promotes the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells
Schrade K , Tröger J, Eldahshan A, Zühlke K, Abdul Azeez KR, Elkins JM , Neuenschwander M , Oder A, Elkewedi M, Jaksch S, Andrae K, Li J, Fernandes J, Müller PM, Grunwald S, Marino SF, Vukićević T, Eichhorst J, Wiesner B, Weber M, Kapiloff M, Rocks O, Daumke O, Wieland T, Knapp S, von Kries JP, Klussmann E
PLoS One, 2018 Jan 26;13(1)
Quantitative GTPase Affinity Purification Identifies Rho Family Protein Interaction Partners
Paul F, Zauber H, von Berg L, Rocks O, Daumke O, Selbach M
Mol Cell Proteomics, 2017 Jan;16(1):73-
Bimodal antagonism of PKA signalling by ARHGAP36
Eccles RL, Czajkowski MT, Barth C, Müller PM, McShane E, Grunwald S, Beaudette P, Mecklenburg N, Volkmer R, Zühlke K, Dittmar G, Selbach M, Hammes A, Daumke O, Klussmann E, Urbé S, Rocks O.
Nat Commun, 2016 Oct 7;7:12963
Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers
Arumughan A, Roske Y, Barth C, Forero LL, Bravo-Rodriguez K, Redel A, Kostova S, McShane E, Opitz R, Faelber K, Rau K, Mielke T, Daumke O, Selbach M, Sanchez-Garcia E, Rocks O, Panáková D, Heinemann U, Wanker EE
Nat Commun, 2016 Oct 20;7:13047.
The anticancer phytochemical rocaglamide inhibits Rho GTPase activity and cancer cell migration
Becker MS, Müller PM, Bajorat J, Schroeder A, Giaisi M, Amin E, Ahmadian MR, Rocks O, Köhler R, Krammer PH, Li-Weber M
Oncotarget, 2016 Aug 9;7(32):51908-51921
A new role of the Rac-GAP b2-chimaerin in cell adhesion reveals opposite functions in breast cancer initiation and tumor progression
Casado-Medrano V, Barrio-Real L, García-Rostán G, Baumann M, Rocks O, Caloca MJ
Oncotarget, 2016 May 10;7(19):28301-19
Src homology 2 domain containing protein 5 (SH2D5) binds the breakpoint cluster region protein, BCR, and regulates levels of Rac1-GTP
Gray EJ, Petsalaki E, James DA, Bagshaw RD, Stacey MM, Rocks O, Gingras AC, Pawson T
J Biol Chem, 2014 Dec 19;289(51):35397-408
Structural insights into oligomerization and mitochondrial remodelling of dynamin 1-like protein
Fröhlich C, Grabiger S, Schwefel D, Faelber K, Rosenbaum E, Mears J, Rocks O, Daumke O
EMBO J, 2013 May 2;32(9):1280-92
Structural insights into the mechanism of GTPase activation in the GIMAP family
Schwefel D, Arasu BS, Marino SF, Lamprecht B, Köchert K, Rosenbaum E, Eichhorst J, Wiesner B, Behlke J, Rocks O, Mathas S, Daumke O
Structure, 2013 Apr 2;21(4):550-9
A roadmap to generate renewable protein binders to the human proteome
Colwill K; Renewable Protein Binder Working Group, Gräslund S
Nat Methods, 2011 May 15;8(7):551-8
Regulation of Ras localization by acylation enables a mode of intracellular signal propagation
Lorentzen A, Kinkhabwala A, Rocks O, Vartak N, Bastiaens PI
Sci Signal, 2010 Sep 21;3(140) F1000 recommended
Small-molecule inhibition of APT1 affects Ras localization and signaling
Dekker FJ*, Rocks O*, Vartak N, Menninger S, Hedberg C, Balamurugan R, Wetzel S, Renner S, Gerauer M, Schölermann B, Rusch M, Kramer JW, Rauh D, Coates GW, Brunsveld L, Bastiaens PI, Waldmann H
Nat Chem Biol, 2010 Jun;6(6):449-56 *Equal contributions
The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins
Rocks O, Gerauer M, Vartak N, Koch S, Huang ZP, Pechlivanis M, Kuhlmann J, Brunsveld L, Chandra A, Ellinger B, Waldmann H, Bastiaens PI
Cell, 2010 Apr 30;141(3):458-71
Spatio-temporal segregation of Ras signals: one ship, three anchors, many harbors
Rocks O, Peyker A, Bastiaens PI
Curr Opin Cell Biol, 2006 Aug;18(4):351-7
An acylation cycle regulates localization and activity of palmitoylated Ras isoforms
Rocks O, Peyker A, Kahms M, Verveer PJ, Koerner C, Lumbierres M, Kuhlmann J, Waldmann H, Wittinghofer A, Bastiaens PI
Science, 2005 Mar 18;307(5716):1746-52 F1000 recommended
Imaging activation of two Ras isoforms simultaneously in a single cell
Peyker A, Rocks O, Bastiaens PI
Chembiochem, 2005 Jan;6(1):78-85
Red-edge anisotropy microscopy enables dynamic imaging of homo-FRET between green fluorescent proteins in cells
Squire A, Verveer PJ, Rocks O, Bastiaens PI
J Struct Biol, 2004, Jul;147(1):62-9
Imaging protein interactions by FRET microscopy: FLIM measurements
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc, 2006 Nov 1;2006(6)
Imaging protein interactions by FRET microscopy: FRET measurements by acceptor photobleaching
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc, 2006 Nov 1;2006(6)
Imaging protein interactions by FRET microscopy: FRET measurements by sensitized emission
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc, 2006 Nov 1;2006(6)
FLIM measurements and frequency domain FLIM data analysis
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc, 2006 Nov 1;2006(6)
An experimental setup for frequency domain FLIM
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc, 2006 Nov 1;2006(6)
Measuring FRET by acceptor photobleaching
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc. 2006 Nov 1;2006(6)
Measuring FRET by sensitized emission
Verveer PJ, Rocks O, Harpur AG, Bastiaens PI
CSH Protoc, 2006 Nov 1;2006(6)
EGFR activation coupled to inhibition of tyrosine phosphatases causes lateral signal propagation
Reynolds AR, Tischer C, Verveer PJ, Rocks O, Bastiaens PI.
Nat Cell Biol, 2003 May;5(5):447-53
Structural domains required for channel function of the mouse transient receptor potential protein homologue TRP1beta
Engelke M, Friedrich O, Budde P, Schäfer C, Niemann U, Zitt C, Jüngling E, Rocks O, Lückhoff A, Frey J. FEBS Lett, 2002 Jul 17;523(1-3):193-9